NGS Library Preparation

Discover our Different NGS Library Preparation Techniques

NGS library preparation is the process of converting a DNA sample into a fragment library suitable for next-generation sequencing. This essential step makes the DNA compatible with sequencing platform requirements and enables accurate downstream analysis.

In our laboratory, we use several NGS library preparation methods to add universal adapter sequences and sample-specific index sequences to the ends of DNA fragments. These adapters support sequencing, while the index sequences allow sample identification and multiplexing in a single run.

Shotgun library preparation

A fragment library is generated from the entire DNA sample without targeting specific regions. This type of shotgun library preparation is suitable for whole genome sequencing and is an efficient, cost-effective, and rapid way to characterize microbiome communities.

Amplicon library preparation

Amplicon library preparation makes previously PCR-amplified DNA fragments suitable for sequencing.

Single-cell

We also use technologies that enable characterization of the transcriptome profile of a single cell or of small cell populations without prior RNA isolation.

mRNA

RNA sequencing is a powerful method for the qualitative and quantitative analysis of the human transcriptome and regulatory RNAs. For bulked mRNA libraries, the mRNA population is specifically selected from the samples by poly-A selection after total RNA isolation. Following reverse transcription, an NGS library preparation workflow is used to generate the library from cDNA. This method enables detection and quantification of even rare coding transcript variants.

Total RNA

When the goal is to study more than gene expression patterns, it is possible to generate a total RNA library by selectively removing ribosomal RNAs through ribodepletion, leaving the RNAs of interest for analysis.

miRNA

miRNA library construction for microRNA analysis involves rigorous size-selection steps that enable sequencing and study of small regulatory RNAs from total RNA samples.

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