Shotgun library preparation:

A fragment library is created from the entire DNA sample without targeting a shotgun library.

This library type is suitable for whole genome sequencing and is an excellent means to characterise microbiome communities cost-effectively and quickly.

Amplicon library preparation:

It is possible to make previously prepared, PCR amplification-derived DNA fragments suitable for sequencing.

Single-cell:

We also use technologies that enable the characterisation of the transcriptome profile of a single cell or populations of limited cell numbers without prior RNA isolation.

mRNA

RNA sequencing is a powerful method for the qualitative and quantitative analysis of the human transcriptome and regulatory RNAs. As for “bulked mRNA” libraries, the mRNA population is specifically selected from the samples (poly-A selection) after total RNA isolation, and, following reverse transcription, an NGS library is prepared from the cDNA. This method allows for detecting and quantifying even rare coding transcript variants.

Total RNA

 When we are interested in more than gene expression patterns, it is possible to generate an entire RNA library by specifically removing ribosomal RNAs (ribodepletion)st of the RNAs that are irrelevant that make up mo for analysis.

miRNA

 miRNA library construction for the analysis of microRNAs involves rigorous size selection steps that specifically enable the sequencing and, thus, the study of small regulatory RNAs from total RNA samples.