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NGS library preparation techniques
The DNA sample is converted into a fragment library suitable for next-generation sequencing during NGS library preparation. In our laboratory, we use several methods for this process which you can find the details of below
PCR techniques
Design of specific oligo pairs for the PCR amplification of a given DNA region and the optimization of the amplification.
Amplicon-based NGS analyses
Amplicon libraries can be used to target a previously identified variant, e.g., to detect the emergence of mutations conferring resistance to anti-tumour treatment.
Hybridisation Capture-Based NGS Panel tests
Hybridisation panel tests allow us to sequence whole genes, perform CNV analysis, and examine fusions. With hybridisation libraries, we can simultaneously analyse up to several hundred genes.
bringing your Research to life
No matter the stage of your research project, we can build a custom plan to help bring your product to market, scale research production, or bespoke one-off production cycles, all in GMP compliant facilities with over 5 decades of experience on staff.