PCR Techniques

Primer design

Design of specific oligo pairs for the PCR amplification of a target DNA region and optimization of the amplification.

Plain PCR

In vitro amplification of a target DNA fragment without cloning, from any sample from which DNA can be isolated or that we receive as purified DNA.

Lactose intolerance testing

PCR-based analysis of DNA isolated from mucosal samples for lactose intolerance testing.

BRCA-targeted mutation testing

PCR-based detection of mutations in the BRCA1 and BRCA2 genes using primers designed for the corresponding regions.

PCR for sequencing

Pre-sequencing PCR for capillary sequencing of samples.

Multiplex PCR

PCR amplification of multiple target regions in a single reaction.

qPCR

Real-time quantitative PCR for measuring the PCR product generated after each cycle. Detection is based on fluorescent dyes or probes, depending on the application.

Probe-based qPCR

Specific probe-based qPCR, such as TaqMan-based detection, for more detailed analysis and real-time monitoring of PCR amplification. The LightCycler® 480 (Roche) real-time PCR instrument is routinely used in our laboratory.

Capillary sequencing

Sanger sequencing of single DNA isolates, PCR products, or plasmids using the ABI 3500 Genetic Analyzer capillary sequencer.

Fragment analysis

Separation of DNA fragments by length after specific labelling PCR, using the ABI 3500 Genetic Analyzer with resolution of up to 1 nt.

Analysis of formalin-fixed paraffin-embedded samples

DNA isolation and analysis from formalin-fixed paraffin-embedded samples using the technology of choice.